Spain had won eight games in a row before this defeat
Spain lost for the first time since they were crowned world champions as Italy produced a surprise win in the Women's Nations League.
Athenea del Castillo put the hosts ahead in Pontevedra before Italy scored three times in 18 second-half minutes.
Michela Cambiaghi put the visitors ahead after Valentina Giacinti's equaliser, with former Atletico Madrid defender Elena Linari adding the third.
Esther Gonzalez got Spain's second but they were unable to find an equaliser.
Despite defeat, Spain have qualified for the Nations League finals with a game to spare.
It was their first defeat since losing 4-0 to Japan at the World Cup on 31 July in Wellington, New Zealand.
They had won eight games in a row, including a 1-0 victory over England in the World Cup final, before losing to Italy.
- Line-upsMatch Stats
Line-upsSpain
Formation 4-3-3
23Coll2Batlle4Paredes14Aleixandri19Carmona6Bonmatí3Abelleira10Hermoso22del Castillo7Paralluelo8Caldentey Oliver
- 23Coll2Batlle4ParedesSubstituted forAndrésat 68'minutesBooked at 83mins14Aleixandri19Carmona6BonmatíSubstituted forGonzálezat 45'minutes3Abelleira10Hermoso22del CastilloSubstituted forGarcíaat 45'minutesBooked at 85mins7Paralluelo8Caldentey Oliver
Substitutes
- 1Rodríguez Rivero5Andrés9González11Pérez12Hernández13Salón15Navarro16Codina17García18Oroz20Benítez21Gabarro
Italy
Formation 5-4-1
1Giuliani2Bergamaschi13Di Guglielmo19Lenzini5Linari17Boattin21Cambiaghi6Giugliano18Caruso16Dragoni9Giacinti
- 1Giuliani2Bergamaschi13Di GuglielmoSubstituted forSalvaiat 90+1'minutes19Lenzini5Linari17Boattin21CambiaghiSubstituted forPiemonteat 90+2'minutes6GiuglianoSubstituted forGalliat 71'minutes18Caruso16DragoniSubstituted forCatenaat 55'minutes9GiacintiSubstituted forCantoreat 55'minutes
Substitutes
- 3Gama4Galli7Cantore8Piemonte10Catena11Bonfantini12Schroffenegger14Severini15Serturini20Greggi22Baldi23Salvai
Referee:Eleni AntoniouMatch StatsHome TeamSpainAway TeamItalyPossessionHome70%Away30%ShotsHome14Away5Shots on TargetHome6Away4CornersHome11Away4FoulsHome11Away7
Source: bbc.com